Application for: Radio Longo | Last updated: August 5, 2021
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Microbiological monitoring is generally performed to assess the microbial concentration in the air and on surfaces in work environments (materials, tools, equipment, clothing).
Which technique is widely used in hospitals for microbiological monitoring of indoor air?
The control of microbiological pollution in the workplace can be carried out by filtration. Filtration sampling is a widely used method for aerosols of chemical origin, and has been adapted for microbiological monitoring.
What is the main purpose of environmental and biological monitoring?
Environmental microbiological monitoring aims to estimate airborne environmental contamination and surface contamination.
What is an indicator of the air quality in the workplace?
The airborne microbial concentration is an index of the air quality in the environment while the contamination of the surfaces can be indicative of the effectiveness of the cleaning and sanitizing procedures.
What is environmental monitoring for?
Environmental monitoring is defined by the European Environment Agency (EEA) as “the measurement, evaluation and determination of environmental parameters and / or pollution levels, periodic and / or continuous in order to prevent negative and harmful effects on the environment”.
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How is the bacterial load determined?
The bacterial load can be estimated by counting the colony-forming units present on the Petri dish where a known volume of the liquid to be analyzed has been grown.
How to destroy bacterial spores?
For some spores, hydrogen peroxide (H2O2) and phenylacetic acid (PAA) are the best choices; for other spores, sodium hypochlorite (NaOCl) may be more effective against the spores, but may not be compatible with the substrate and with the presence of organic material which could rapidly inactivate the …
What does colony-forming units mean?
In microbiology, the so-called “colony forming unit” (CFU) is the unit used to estimate the number of viable bacteria or fungal cells present in a sample, and is defined as the ability to multiply by binary fission under controlled conditions.
How to calculate CFU ml?
calculate the colony forming units (cfu) per milliliter with the following formula: cfu / ml = n ° colonies I plate + n ° colonies II plate x Dil 2 where Dil. = 10 or 100 or 1000, etc. depending on the dilution of the plates used for counting.
How are bacteria counted?
The use of membrane filters is another good method for measuring the bacterial population. The sample is first filtered through cellulose filter membranes, which are subsequently placed in a plate with suitable medium. After incubation, the colonies present can be observed and counted.
What is the purpose of counting microorganisms?
The count of aerobic microorganisms at 30 ° C (according to the ISO 4833 series) is one of the parameters most used by the food industry to evaluate, for example, the adequacy of good hygiene practices and any post-process recontamination.
Which cleanser is effective against spores?
Alternatively, propyl alcohol can also be used. sodium hypochlorite, available on the market in concentrations ranging between 1.5 and 15%, has an efficient disinfectant action against bacteria, viruses, molds and spores even at low concentrations.
What treatment eliminates the spores?
Sterilization (V level): killing of all microorganisms including spores.
At what temperature are bacterial spores killed?
Most bacteria are killed in 5-10 minutes at 60-70 ° C (with moist heat). Bacterial spores are killed at higher temperatures, often above 100 ° C.
What is meant by virulence?
virulence Degree of pathogenic potential possessed by a microorganism, ie its ability to induce pathological manifestations.
What does non-significant bacterial load mean?
In fact, contamination can occur at the time of emission or collection, in which case we speak of bacteriurian not significant. It is considered pathological, that is, a sign of a low (bladder) or high (kidney) infection, only in the presence of more than 10,000 bacteria per ml of urine.
What preventive measures can be taken to avoid bacterial contamination of milk?
Protect food with cabinets, displays, counters, to prevent any contamination even from customers. Avoid touching food with your hands, instead using forks, spoons, spatulas, napkins, paper and other items suitable for the purpose.
What does sterilization eliminate?
It is a technique that exploits the action of flowing (Koch’s pot) or saturated (autoclave) steam; it eliminates microorganisms by denaturating their proteins and other biomolecules.
What is destroyed by sterilization?
Definition: sterilization is a process capable of destroying all vegetative forms (pathogenic and non-pathogenic bacteria) including spores.
Which process eliminates the pathogenic microorganisms and not the spores?
DISINFECTION = process that reduces or completely eliminates all pathogenic microorganisms in the vegetative state. It is not active against spores.
What are the strongest disinfectants?
The types of disinfectant
- Bleach. One of the most popular and inexpensive products is bleach. …
Alcohol-based disinfectants. Alcohol is widely known for its antiseptic and disinfectant properties. …
- Hydrogen peroxide. …
- Neutral soap and water.
What are the various types of disinfectants?
Types of Disinfectants
- Halogenated compounds. Among the disinfectants containing halogens in their chemical structure, certainly the best known are hypochlorites and, in particular, sodium hypochlorite (commonly known as “bleach”). …
- Alcohols. …
- Aldehydes. …
- Heterocyclic compounds.
How to disinfect Covid?
The WHO indications suggest the use of products normally used in cleaning and disinfection even in pre-COVID times, such as the common 0.1% bleach or alcohol at a concentration of 70%.
How is the generation time calculated?
The generation time, g, of the cell population is calculated as t / n, where t is the time in hours or minutes of exponential growth.
How much is a colony?
A bacterial colony consists of approximately 106-107 cells, which come about 20-25 generations from the initial cell.
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